Overview

This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Microbial Transposon Mutagenesis: Protocols and Applications serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.

Full Product Details

Author:   Steven C. Ricke ,  Si Hong Park ,  Morgan L. Davis
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   2016
Weight:   0.454kg
ISBN:  

9781493995721

ISBN 10:   1493995723
Pages:   183
Publication Date:   14 August 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Preparation of Transposon Library and Tn-Seq Amplicon Library for Salmonella Typhimurium.- Application of Whole Genome Sequencing to Transposon Mutants of Salmonella Heidelberg.- Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus.- Transposon Mutagenesis in Streptococcus Species.- Implementation of Transposon Mutagenesis in Bifidobacterium.- Transposon Mutagenesis of Listeria monocytogenes.- Transposon Mutagenesis of Foodborne Pathogenic Esherichia coli.- Methods for Transposon Mutagenesis in Proteus mirabilis.- Mutagenesis of Vibrio fischeri and Other Marine Bacteria Using Hyperactive Mini-Tn5 Derivatives.- Transposon Mutagenesis of Bacteroides fragilis.- Transposon Mutagenesis in Mycobacterium avium Subspecies Paratuberculosis.- Protocols of Conjugative Plasmid Transfer in Salmonella: Plate, Broth, and Filter Mating Approaches.- Construction of DNA Barcode-Tagged Salmonella Strains.- Transposon-Aided Capture of Antibiotic Resistance Plasmids from Complex Samples.- Efficient Gene Deletion Method for Listeria monocytogenes.- Whole Genome Identification and Characterization of Bacterial Insertion Sequences Using Bioinformatic Tools.

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions