Overview

This second edition provides new and updated chapters describing the utility and attributes of different tools that are used for studying microbial systems. Chapters detail a number of methods, including in silico system level analyses by MetaFlux, Kbase genome-scale model builder, COBRA toolbox, NanoSIP, and PAMMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Microbial Systems Biology: Methods and Protocols, Second Edition aims to introduce and aid scientists in using the various tools that are currently available for analysis, modification, and utilization of microbial organisms.

Full Product Details

Author:   Ali Navid
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2022
Volume:   2349
Weight:   0.758kg
ISBN:  

9781071615874

ISBN 10:   1071615874
Pages:   385
Publication Date:   31 October 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Parallel Accelerator and Molecular Mass Spectrometry Measurement of Carbon-14 Labeled Analytes.- Fast sampling of the cellular metabolome.- Investigation of Protein-Lipid Interactions Using Native Mass Spectrometry.- Western Blot Processing Optimization: The Perfect Blot.- FISHing on a Budget.- NanoSIP: NanoSIMS Applications for Microbial Biology.- Construction of Metatranscriptomic Libraries for 5' end Sequencing of rRNAs for Microbiome Research.- Computational Approaches for Designing Highly Specific and Efficient sgRNAs.- Complex Network Analysis in Microbial Systems: Theory and Examples.- Prokaryotic Genome Annotation.- Functional Annotation from Structural Homology.- Metabolic Modeling with MetaFlux.- Application of the Metabolic Modeling Pipeline in KBase to Categorize Reactions, Predict Essential Genes, And Predict Pathways in an Isolate Genome.- Curating COBRA Models of Microbial Metabolism.- A Beginner’s Guide to the COBRA Toolbox.- Rules of Engagement: A Guide to Developing Agent-Based Models.

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