Overview

Outstanding experts in the use of microorganisms as cell factories describe in step-by-step detail their best laboratory procedures for a wide range of processes and products mediated by microorganisms. The diverse chemicals and biochemicals produced can be used in human health, nutrition, and environmental protection, and include semisynthetic derivatives of cephalosporins, erythromycin, antitumor compounds, plasmids for gene therapy and DNA vaccination , L-lysine, Vitamins B2 and B12, the sweet-tasting protein thaumatin, the carotenoids ss-carotene and astaxanthin, the polysaccharide gellan, and bacteria-producing bacteria for sausage fermentation, and many more. Also illustrated are the uses of phenylacetyl-CoA catabolon for the enzymatic synthesis of penicillins, aromatic biotransformation, syntheses of novel bioplastics, biosensor design, and the synthesis of drug vehicles. This book offers laboratory and industrial scientists a wealth of readily reproducible techniques for the microbial generation of biochemical products serving human health, nutrition, and environmental protection.

Full Product Details

Author:   José-Luis Barredo
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2005
Volume:   18
Dimensions:   Width: 15.20cm , Height: 2.70cm , Length: 22.90cm
Weight:   0.894kg
ISBN:  

9781617376351

ISBN 10:   1617376353
Pages:   516
Publication Date:   19 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Development of Improved Strains and Optimization of Fermentation Processes.- Experimental Design in Microbiology.- Metabolic Engineering of Acremonium chrysogenum to Produce Deacetoxycephalosporin C and Bioconversion to 7-Aminodeacetoxycephalosporanic Acid.- Production of Erythromycin With Saccharopolyspora erythraea.- The Phenylacetyl-CoA Catabolon.- Recombinant Microorganisms for the Biosynthesis of Glycosylated Antitumor Compounds.- Assay Methods for Detection and Quantification of Antimicrobial Metabolites Produced by Streptomyces clavuligerus.- Purification of Plasmid DNA Vectors Produced in Escherichia coli for Gene Therapy and DNA Vaccination Applications.- Genome Breeding of an Amino Acid-Producing Corynebacterium glutamicum Mutant.- Metabolic Activity Profiling by 13C Tracer Experiments and Mass Spectrometry in Corynebacterium glutamicum.- Protein and Vitamin Production in Bacillus megaterium.- Strategies for Large-Scale Production of Recombinant Proteins in Filamentous Fungi.- Strain and Culture Conditions Improvement for ?-Carotene Production With Mucor.- Xanthophylls in Fungi.- Methodologies for the Analysis of Fungal Carotenoids.- Insertional Mutagenesis in the Vitamin B2 Producer Fungus Ashbya gossypii.- Improved Polysaccharide Production Using Strain Improvement.- Use of the Morganella morganii phoC Gene as Reporter in Bacterial and Yeast Hosts.- Gene Expression Arrays in Food.- Optimization of Proteome Analysis for Wine Yeast Strains.- Bacteriocin-Producing Strains in a Meat Environment.- In Vitro and In Vivo Interactions of Nonpathogenic Bacteria With Immunocompetent Cells.- Volatile Sulfur Detection in Fermented Foods.- Bioleaching.- Use of PhoN Phosphatase to Remediate Heavy Metals.- Safe Dispatch and Transport of Biological Material.- How to DepositBiological Material for Patent Purposes.- Laws and Regulations for the Protection of Biotechnological Inventions.

Reviews

Microbial Processes and Products is the latest in the series Methods in Biotechnologya , some issues of which have been highly focused and widely relevant (such as Enzymes in Nonaqueous Solvents, Vulfson et al., 2001). This volume (and indeed the entire series) is targeted to the research community. Those researchers finding a relevant chapter in this text are likely to be rewarded. As the series title suggests, this is a 'methods' volume and the sections are packed with detailed protocols, tips and useful practical advice. The text is generally readable and the chapters are well referenced. -Don Cowan, University of the Western Cape ...the sections are packed with detailed protocols, tips, and useful practical advice. - Microbiology Today

Microbial Processes and Products is the latest in the series Methods in Biotechnology(t), some issues of which have been highly focused and widely relevant (such as Enzymes in Nonaqueous Solvents, Vulfson et al., 2001). This volume (and indeed the entire series) is targeted to the research community. Those researchers finding a relevant chapter in this text are likely to be rewarded. As the series title suggests, this is a 'methods' volume and the sections are packed with detailed protocols, tips and useful practical advice. The text is generally readable and the chapters are well referenced. -Don Cowan, University of the Western Cape ...the sections are packed with detailed protocols, tips, and useful practical advice. - Microbiology Today

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