Microbial Metabolic Engineering: Methods and Protocols

Author:   Christine Nicole S. Santos ,  Parayil Kumaran Ajikumar
Publisher:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1927
ISBN:  

9781493991419


Pages:   252
Publication Date:   21 February 2019
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Microbial Metabolic Engineering: Methods and Protocols


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Author:   Christine Nicole S. Santos ,  Parayil Kumaran Ajikumar
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1927
Weight:   0.730kg
ISBN:  

9781493991419


ISBN 10:   1493991418
Pages:   252
Publication Date:   21 February 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Pathway and Gene Discovery from Natural Hosts and Organisms Jing Ke and Yasuo Yoshikuni 2. Metabolic In silico Network Expansions to Predict and Exploit Enzyme Promiscuity James Jeffryes, Jonathan Strutz, Chris Henry, and Keith E.J. Tyo 3. Antisense RNA Elements for Downregulating Expression Yaping Yang, Jian Wang, Ruihua Zhang, and Yajun Yan 4. Chemically Inducible Chromosomal Evolution (CIChE) for Multi-copy Metabolic Pathway Engineering Aaron M. Love, Bradley W. Biggs, Keith E.J. Tyo, and Parayil Kumaran Ajikumar 5. Bacterial Expression of Membrane Associated Cytochrome P450s and their Activity Assay in Nanodiscs Demetrios Maroutsos, Hannah Huff, and Aditi Das 6. Delta Integration CRISPR-Cas (Di-CRISPR) in Saccharomyces cerevisiae Shuobo Shi, Youyun Liang, Ee Lui Ang, and Huimin Zhao 7. Assembly of Complex Pathways using Type IIs Restriction Enzymes Sylvestre Marillonnet and Stefan Werner 8. Combinatorial Assembly of Multigene Pathways by Combining Single Strand Assembly with Golden Gate Assembly D. Bauwens, P. Coussement, J. Maertens, and M. De Mey 9. Checks and Balances with Use of the Keio Collection for Phenotype Testing Sandra J. Aedo, Helena R. Ma, and Mark P. Brynildsen 10. Genetic Engineering of Cyanobacteria: Design, Implementation, and Characterization of Recombinant Synechocystis sp. PCC 6803 Jacob Sebesta, Allison Zimont, and Christie Ann Marie Peebles 11. Genetic Tools for Streamlined and Accelerated Pathway Engineering in Yarrowia lipolytica Lynn Wong, Benjamin Holdridge, Jake Engel, and Peng Xu 12. Gas Chromatography-mass Spectrometry Microbial Metabolomics for Applications in Strain Optimization McKenzie L. Smith, April M. Miguez, and Mark P. Styczynski 13. Targeted Mass Spectrometry-based Proteomics Tools for Strain Optimization Hsine-Chung Tseng and Christine Nicole S Santos 14. A Protocol for the Construction and Curation of Genome-scale Integrated Metabolic and Regulatory Network Models Sriram Chandrasekaran 15. 13C-Fingerprinting and Metabolic Flux Analysis of Microbial Metabolisms Whitney Hollinshead, Lian He, and Yinjie J. Tang 16. A Genetic Screen for the Isolation of Mutants with Increased Flux in the Isoprenoid Pathway of Yeast Manisha Wadhwa andAnand K Bachhawat

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