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OverviewLeading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Overview chapters on microorganisms as a source of metabolic and enzymatic diversity, and on the fast-moving field of enzyme biosensors are presented. Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications. Full Product DetailsAuthor: Jose Luis BarredoPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: Softcover reprint of hardcover 1st ed. 2005 Volume: 17 Dimensions: Width: 15.20cm , Height: 1.80cm , Length: 22.90cm Weight: 0.489kg ISBN: 9781617374548ISBN 10: 1617374547 Pages: 320 Publication Date: 19 November 2010 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: In Print This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsMicrobial Cells and Enzymes A Century of Progress.- Enzyme Biosensors.- Directed Evolution of Enzymes.- L-Glutaminase as a Therapeutic Enzyme of Microbial Origin.- Enzymatic Production of D-Amino Acids.- Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides.- Food-Grade Corynebacteria for Enzyme Production.- Enzymatic Synthesis of (S)-Phenylalanine and Related (S)-Amino Acids by Phenylalanine Dehydrogenase.- Enzymes in Modern Detergents.- Microbial Proteases.- Proteases Produced by Halophilic Bacteria and Archaea.- Microbial Pectinases.- Expression of Microbial Phytases in Yeast Systems and Characterization of the Recombinant Enzymes.- Glucose Dehydrogenase for the Regeneration of NADPH and NADH.- Acetate Kinase From Methanosarcina thermophila, a Key Enzyme for Methanogenesis.- Immobilization of Enzymes by Covalent Attachment.- Preparation of an Industrial Biocatalyst of Penicillin G Acylase on Sepabeads.- Methods for Bioencapsulation of Enzymes and Cells.ReviewsThe first two chapters alone are worth reading to appreciate the range of microbes and their applications in industry. Thereafter, each chapter deals with a particular topic and presents an introduction followed by an accurate set of methods, which are well set out and easy for the specialist to follow. However, this book would be a good addition to a library, although I suspect its comprehensive coverage of the subject will make it too general for personal purchase. -Robert D. Possee, CEH-Oxford Comprehensive and practical, this book offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications. - BioTech International The first two chapters alone are worth reading to appreciate the range of microbes and their applications in industry. Thereafter, each chapter deals with a particular topic and presents an introduction followed by an accurate set of methods, which are well set out and easy for the specialist to follow. However, this book would be a good addition to a library, although I suspect its comprehensive coverage of the subject will make it too general for personal purchase. -Robert D. Possee, CEH-Oxford Comprehensive and practical, this book offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications. - BioTech International Author InformationTab Content 6Author Website:Countries AvailableAll regions |