Overview

The microelectrode technique is today the most widely used method in electrophy- siology. Microelectrodes offer a unique approach to measurements of electrical pa- rameters and ion activities of single cells. Several important breakthroughs in trans- port physiology have arisen from microelectrode studies. Undoubtedly, there is a progressively wide-spread use of conventional and ion-selective microelectrodes. Due to their particular dimension and properties micro electrodes are exclusive- ly applied to measurements on living matter. This must have many consequences to my thoughts on experiments with microelectrodes. In this book, my concern is fo- cussing on the description of an intracellular method that should lead to reliable in- formation on cellular parameters. The methodical basis for any meaningful applica- tion is treated extensively. However, technical perfection and accurate results are not the only concern when working on animals and human beings. Rather, my thoughts are governed by the intellectual and moral mastery of the experimental ap- proach on living subjects. A measurement with microelectrodes usually necessitates the sacrifice of an ani- mal. This is an immense fact, and means that the knowledge gained by the experi- ment must justify the death of a living subject.

Full Product Details

Author:   Daniel Ammann
Publisher:   Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Imprint:   Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Volume:   50
Dimensions:   Width: 17.00cm , Height: 1.90cm , Length: 24.40cm
Weight:   0.719kg
ISBN:  

9783540162223

ISBN 10:   3540162224
Pages:   348
Publication Date:   01 June 1986
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

Classification of Ion-Selective Electrodes.- Natural and Synthetic Neutral Carriers for Membrane Electrodes.- Liquid Membrane Electrodes Based on Neutral Carriers.- Potentiometric Measurements of Ion Activities with Neutral Carrier-Based Electrodes.- Construction of Liquid Membrane Microelectrodes.- Use of Neutral Carrier-Based Liquid Membrane Microelectrodes.- General Aspects of Intracellular Measurements of Ions.- Neutral Carrier-Based Liquid Membrane Microelectrodes.- Impact of Neutral Carrier Microelectrodes.- References.

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions