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OverviewA fifteen different biphasic media were used to cultivated promastigote parasite of Leishmania donovani and compared with ordinary NNN medium, to find a cow milk agar medium can provide a good cultivation to the parasite as well as the ordinary medium, where milk is a simple, cheap and a good source of protein, carbohydrate, lipids and calcium. But milk cannot be a substituted for rabbit blood as solid phase that was given the greatest count of parasites when using it. Also, using a 10% of human urine as a liquid phase can enhance the growth rate of parasite up to eight times, this reduced the time that needed to cultivate the parasite in the ordinary NNN medium. So, using of a modified NNN medium BHU (medium that used the rabbit blood as solid phase and 10% of human urine as liquid phase) in the field of laboratory can provide a short duration to cultivate Leishmania parasite. Full Product DetailsAuthor: El-Sayed Abdullatif Al-Sayed SohaPublisher: LAP Lambert Academic Publishing Imprint: LAP Lambert Academic Publishing Dimensions: Width: 15.20cm , Height: 0.40cm , Length: 22.90cm Weight: 0.104kg ISBN: 9783659641503ISBN 10: 3659641502 Pages: 64 Publication Date: 28 November 2014 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In stock We have confirmation that this item is in stock with the supplier. It will be ordered in for you and dispatched immediately. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |