In vitro Cultivation of Leishmania donovani in NNN and Milk Agar Media

Author:   El-Sayed Abdullatif Al-Sayed Soha
Publisher:   LAP Lambert Academic Publishing
ISBN:  

9783659641503


Pages:   64
Publication Date:   28 November 2014
Format:   Paperback
Availability:   In stock   Availability explained
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In vitro Cultivation of Leishmania donovani in NNN and Milk Agar Media


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Overview

A fifteen different biphasic media were used to cultivated promastigote parasite of Leishmania donovani and compared with ordinary NNN medium, to find a cow milk agar medium can provide a good cultivation to the parasite as well as the ordinary medium, where milk is a simple, cheap and a good source of protein, carbohydrate, lipids and calcium. But milk cannot be a substituted for rabbit blood as solid phase that was given the greatest count of parasites when using it. Also, using a 10% of human urine as a liquid phase can enhance the growth rate of parasite up to eight times, this reduced the time that needed to cultivate the parasite in the ordinary NNN medium. So, using of a modified NNN medium BHU (medium that used the rabbit blood as solid phase and 10% of human urine as liquid phase) in the field of laboratory can provide a short duration to cultivate Leishmania parasite.

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Author:   El-Sayed Abdullatif Al-Sayed Soha
Publisher:   LAP Lambert Academic Publishing
Imprint:   LAP Lambert Academic Publishing
Dimensions:   Width: 15.20cm , Height: 0.40cm , Length: 22.90cm
Weight:   0.104kg
ISBN:  

9783659641503


ISBN 10:   3659641502
Pages:   64
Publication Date:   28 November 2014
Audience:   General/trade ,  General
Format:   Paperback
Publisher's Status:   Active
Availability:   In stock   Availability explained
We have confirmation that this item is in stock with the supplier. It will be ordered in for you and dispatched immediately.

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