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OverviewPhage Q protein is a transcriptional antiterminator that is required for late gene expression from the pR' promoter when prophage decides to follow the lytic pathway. Q modifies Escherichia coli RNA polymerase (RNAP) at the early elongation stage. Once engaged, Q maintains itself as a subunit of the elongation complex throughout the elongation. Studies show that Q confers RNAP resistance to both intrinsic and -mediated terminators and less pausing. However, little is known about the interaction between Q and RNAP. In this study, the largest subunits ( and ') of RNAP were examined to map the Q binding site. A stronger binding was observed with than ', and the binding site was narrowed to 501-832. This region was further defined as small as 82 amino acids (600-681) that interacted with Q. In addition, 12 mutant RNAPs were isolated for a reduced Q antitermination within the potential Q binding regions, specifically near the main channel. These results propose that Q modifies the active center of RNAP for antitermination. Full Product DetailsAuthor: Heeyoun BunchPublisher: VDM Verlag Dr. Muller Aktiengesellschaft & Co. KG Imprint: VDM Verlag Dr. Muller Aktiengesellschaft & Co. KG Dimensions: Width: 15.20cm , Height: 1.00cm , Length: 22.90cm Weight: 0.267kg ISBN: 9783639165814ISBN 10: 3639165810 Pages: 176 Publication Date: 07 June 2009 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In Print This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |