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Author:   Nicola A. Burgess-Brown
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2017
Volume:   1586
Dimensions:   Width: 17.80cm , Height: 2.50cm , Length: 25.40cm
Weight:   9.732kg
ISBN:  

9781493968855

ISBN 10:   1493968858
Pages:   429
Publication Date:   04 May 2017
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Recombinant Protein Expression in E. coli: A Historical Perspective.- N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools.- Harnessing the Profinity eXactTM System for Expression and Purification of Heterologous Proteins in E. coli.- ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences.- Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters.- Optimization of Membrane Protein Production Using Titratable Strains of E. coli.- Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) or pReX and GFP-Fusions.- High Yield of Recombinant Protein in Shaken E. coli Cultures with Enzymatic Glucose Release Medium EnPresso B.- A Generic Protocol for Purifying Disulphide-Bonded Domains and Random Protein Fragments Using FusionProteins with SUMO3 and Cleavage by SenP2 Protease.- A Strategy for Production of Correctly Folded Disulfide-Rich Peptides in the Periplasm of E. coli.- Split GFP Complementation as Reporter of Membrane Protein Expression and Stability in E. coli: A Tool to Engineer Stability in a LAT Transporter.- Acting on Folding Effectors to Improve Recombinant Protein Yields and Functional Quality.- Protein Folding Using a Vortex Fluidic Device.- Removal of Affinity Tags with TEV Protease.- Generation of Recombinant N-Linked Glycoproteins in E. coli.- Production of Protein Kinases in E. coli.- Expression of Prokaryotic Integral Membrane Proteins in E. coli.- Multiprotein Complex Production in E. coli: The SecYEG-SecDFYajC-YidC Holotranslocon.- Membrane Protein Production in E. coli Lysates in Presence of Pre-Assembled Nanodiscs.- Not Limited to E. coli: Versatile Expression Vectors for Mammalian Protein Expression.- A Generic Protocol for Intracellular Expression of Recombinant Proteins in Bacillus subtilis.- In Vivo Biotinylation of Antigens in E. coli.- Cold-Shock Expression System in E. coli for Protein NMR Studies.- High-Throughput Production of Proteins in E. coli for Structural Studies.- Mass Spectrometric Analysis of Proteins.- How to Determine Interdependencies of Glucose and Lactose Uptake Rates for Heterologous Protein Production with E. coli.- Interfacing Biocompatible Reactions with Engineered Escherichia coli.

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