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OverviewThe dutch elm tree disease casual agent, Ophiostoma ulmi, was investigated for its ability to produce exopolyssacharides (EPS), which have great industrial potential. This book introduced the study of EPS biosynthesis, using proteomics tools with the purpose of identifying the proteins/enzymes that were responsible for EPS biosynthesis. The extracellular O. ulmi protein profile under various culture conditions was obtained with SDS PAGE and then compared. The gel bands were then excised and treated with trypsin, followed by protein identification by a matrix assisted laser desorption/ionization time of flight (MALDI-TOF) instrument. It was found that sucrose synthase, acyl carriers proteins and various transferases were induced by substrate (sucrose), or nitrogen source (asparagine), which indicated an intracellular biosynthesis pathway. This is the first proteomic work based on O. ulmi, which provides insights on both fungal proteomics and fungal EPS biosynthesis. Full Product DetailsAuthor: Chun Bei Huang , And Bradley a SavillePublisher: LAP Lambert Academic Publishing Imprint: LAP Lambert Academic Publishing Dimensions: Width: 15.20cm , Height: 0.60cm , Length: 22.90cm Weight: 0.159kg ISBN: 9783838305011ISBN 10: 3838305019 Pages: 100 Publication Date: 30 May 2010 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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