Overview

A collection of readily reproducible techniques for determining most of the important factors governing microorganisms and their habitats. Presented in step-by-step detail, these cutting-edge methods range from those for the study of marine organisms, to those for investigating microorganisms occurring in groundwater, to the biodiversity of yeasts found in remote environments. The novel protocols for studying fermented milks are significant for investigators concerned with milk as an item of food for infants, small children, and even adults. Additional methods for the recovery and determination of nucleic acids and other compounds affecting, and affected by, microorganisms are given for certain enzymes produced by plant pathogens and for obtaining microbial species tolerant of such inhibitors as heavy metals.

Full Product Details

Author:   John F. T. Spencer ,  Alicia L. Ragout de Spencer
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2004
Volume:   16
Dimensions:   Width: 15.20cm , Height: 2.40cm , Length: 22.90cm
Weight:   0.643kg
ISBN:  

9781617373701

ISBN 10:   1617373702
Pages:   426
Publication Date:   09 December 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Communities and Biofilms.- Isolation and Molecular Characterization of Seawater Bacteria.- Reverse Sample Genome Probing to Monitor Microbial Communities.- T-RFLP Analysis.- Assessing Bacterial DNA Content in Aquatic Systems.- Multiplexed Identification and Quantification of Analyte DNAs in Environmental Samples Using Microspheres and Flow Cytometry.- Molecular Characterization of Microbial Communities From Marine Environments.- Yeasts.- Aeromonads in Environmental Waters.- Development of a Vital Fluorescent Staining Method for Monitoring Bacterial Transport in Subsurface Ground Water.- Bench Scale Flow Cell for Nondestructive Imaging of Biofilms.- Fermented Milks.- ?-Galactosidase Assay in Fermented Soymilk Products.- Considerations to Avoid the Overestimation of Exopolysaccharides Produced by Lactic Acid Bacteria.- Determination of Oligosaccharides in Fermented Soymilk Products by High-Performance Liquid Chromatography.- Evaluation of Minimal Nutritional Requirements of Lactic Acid Bacteria Used in Functional Foods.- Nuclei Acids, Recovery, and Determination.- A Simple Method for Obtaining DNA Suitable for RAPD Analysis From Azospirillum.- PCR Fingerprinting of rRNA Intergenic Spacer Regions for Molecular Characterization of Environmental Bacteria Isolates.- A DNA-DNA Hybridization Method for the Detection and Quantification of Specific Bacterial Taxa in Natural Environments.- 13C and 1H NMR Study of the Glycogen Futile Cycle in Fibrobacter succinogenes.- Chemical Analysis and Biological Removal of Wood Lipids Forming Pitch Deposits in Paper Pulp Manufacturing.- Propolis.- Enzymatic Saccharification of Cellulosic Materials.- Molecular Identification of Microbial Populations in Petroleum-Contaminated Groundwater.- Identification of Copper-Resistant Microorganisms byPCR.- Selection of Tolerant Heavy Metal Yeasts From Different Polluted Sites.- Medium for Differential Enumeration of Lactobacillus casei and Lactobacillus acidophilus From Lyophilized Mixed Cultures.- Silk as a Means of Recovering Bacteriocin From a Culture Medium.- Optimization of a Method for Isolating Plasmid DNA From Lactic Acid Bacteria From Wine.- Simultaneous and Sequential Methods to Study Interactions Between Yeast and Lactic Acid Bacteria From Wine.- Method for Determining Lindane Concentration in Water and Solid Samples.- Extracellular Hydrolytic Enzymes Produced by Yeasts.- Extracellular Hydrolytic Enzymes Produced by Phytopathogenic Fungi.- An Analysis of Microorganisms in Environments Using Denaturing Gradient Gel Electrophoresis.- Testing for Evolutionary Correlations in Microbiology Using Phylogenetic Generalized Least Squares.- Conjugated Linoleic Acid Detection Produced by Dairy Starter Cultures.- Reviews.- Techniques for Manipulating the Bacterial Endophyte Bacillus mojavensis.- Engineering of Bacteria Using the Vitreoscilla Hemoglobin Gene to Enhance Bioremediation of Aromatic Compounds.- The Use of Chemical Shift Reagents and 23Na NMR to Study Sodium Gradients in Microorganisms.- Denaturing Gradient Gel Electrophoresis (DGGE) as a Fingerprinting Tool for Analyzing Microbial Communities in Contaminated Environments.

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