Overview

A cutting-edge collection of updated and core techniques for the neurological study of drugs of abuse. Described in step-by-step detail by hands-on experts, these readily reproducible protocols cover a wide variety of coherent methods for gathering information on quantitative changes in protein and mRNA at both tissue and cellular levels. There are various methods for detecting single and multiple alterations in single and multiple gene expression, for analyzing the functional roles of genes and proteins, for studying the release kinetics of striatal dopamine, and for the quantitative measurement of such neurotransmitters as acetylcholine. Insightful review articles address key current topics on the effect of drugs of abuse on the brain.

Full Product Details

Author:   John Q. Wang
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2003
Volume:   79
Dimensions:   Width: 15.20cm , Height: 3.00cm , Length: 22.90cm
Weight:   0.771kg
ISBN:  

9781617373282

ISBN 10:   1617373281
Pages:   514
Publication Date:   09 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Special Reviews.- The Temporal Sequence of Changes in Gene Expression by Drugs of Abuse.- Effects of Psychomotor Stimulants on Glutamate Receptor Expression.- Adult Neural Stem/Progenitor Cells in the Forebrain.- Neuroprotective Effect of Naloxone in Inflammation-Mediated Dopaminergic Neurodegeneration.- Neuropharmacology and Neurotoxicity of 3,4-Methylenedioxymethamphetamine.- Learning and Memory Mechanisms Involved in Compulsive Drug Use and Relapse.- From Drugs of Abuse to Parkinsonism: The MPTP Mouse Model of Parkinson’s Disease.- Detection of MRNA Expression in the Striatum.- In Situ Hybridization with Isotopic Riboprobes for Detection of Striatal Neuropeptide mRNA Expression After Dopamine Stimulant Administration.- Combining In Situ Hybridization with Retrograde Tracing and Immunohistochemistry for Phenotypic Characterization of Individual Neurons.- Analysis of mRNA Expression Using Double In Situ Hybridization Labeling with Isotopic and Nonisotopic Probes.- Quantification of mRNA in Neuronal Tissue by Northern Analysis.- Analysis of Gene Expression in Striatal Tissue by Multiprobe RNase Protection Assay.- Analysis of mRNA Expression in Striatal Tissue by Differential Display Polymerase Chain Reaction.- Semiquantitative Real-Time PCR for Analysis of mRNA Levels.- Application of TaqMan RT-PCR for Real-Time Semiquantitative Analysis of Gene Expression in the Striatum.- Systematic Screening of Gene Expression Using a cDNA Macroarray.- Detection of Protein Expression in the Striatum.- Quantification of Protein in Brain Tissue by Western Immunoblot Analysis.- Immunohistochemical and Immunocytochemical Detection of Phosphoproteins in Striatal Neurons.- Analysis of Protein Expression in Brain Tissue by ELISA.- Dopamine Receptor Binding and QuantitativeAutoradiographic Study.- Analysis of DNA-Binding Activity in Neuronal Tissue with the Electrophoretic Mobility-Shift Assay.- Gene Function Analysis.- Viral-Mediated Gene Transfer to Study the Behavioral Correlates of CREB Function in the Nucleus Accumbens of Rats.- Generating Gene Knockout Mice for Studying Mechanisms Underlying Drug Addiction.- Antisense Approaches in Analyzing the Functional Role of Proteins in the Central Nervous System.- Striatal Culture Preparation.- Primary Striatal Neuronal Culture.- Primary Rat Mesencephalic Neuron-Glia, Neuron-Enriched, Microglia-Enriched, and Astroglia-Enriched Cultures.- Primary Culture of Adult Neural Progenitors.- Organotypic Culture of Developing Striatum.- Detection of Transmitter Release in the Striatum.- Microdialysis Coupled with Electrochemical Detection.- HPLC/EC Detection and Quantification of Acetylcholine in Dialysates.- Real-Time Measurements of Phasic Changes in Extracellular Dopamine Concentration in Freely Moving Rats by Fast-Scan Cyclic Voltammetry.- Measurement of Dopamine Uptake in Neuronal Cells and Tissues.- Behvioral Assessment and Others.- Laboratory Analysis of Behavioral Effects of Drugs of Abuse in Rodents.- Conditioned Place Preference.- Detection of Cell Proliferation and Cell Fate in Adult CNS Using BrdU Double-Label Immunohistochemistry.

Reviews

It describes in detail laboratory methods used by researchers working in the area of drug abuse and drug addiction. This is a unique book. Few books deal in detail with the methods underlying experiments. It will be of primary value to active investigators with active laboratories. -Doody's Health Sciences Book Review Journal

It describes in detail laboratory methods used by researchers working in the area of drug abuse and drug addiction. This is a unique book. Few books deal in detail with the methods underlying experiments. It will be of primary value to active investigators with active laboratories. -Doody's Health Sciences Book Review Journal

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