Free Delivery Over $100
|
|
|||
|
||||
OverviewAn unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs. Described by expert experimentalists who have perfected the techniques, these unfailingly reproducible methods include assays for enzyme-catalyzed DNA relaxation/supercoiling, DNA cleavage, DNA nicking, DNA decatenation, and ATP hydrolysis. Several modified DNA substrates, used to dissect enzyme mechanisms by trapping intermediates, are also described. Methodologies to determine the action of topoisomerase-targeted drugs include biochemical assays of drug-induced enzyme-DNA complexes, methods for assaying drug uptake, and cell-based assays for determining the specificity and mechanisms of drug resistance. A companion volume, DNA Topoisomerase Protocols, I: DNA Topology and Enzymes, provides state-of-the-art experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Full Product DetailsAuthor: Neil Osheroff , Mary-Ann BjornstiPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: Softcover reprint of hardcover 1st ed. 2001 Volume: 95 Dimensions: Width: 15.20cm , Height: 1.90cm , Length: 22.90cm Weight: 0.512kg ISBN: 9781617370571ISBN 10: 1617370576 Pages: 334 Publication Date: 10 November 2010 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsAssaying DNA Topoisomerase I Relaxation Activity.- DNA Topoisomerase II-Catalyzed DNA Decatenation.- Plasmid DNA Supercoiling by DNA Gyrase.- Analyzing Reverse Gyrase Activity.- Topoisomerase II-Catalyzed ATP Hydrolysis as Monitored by Thin-Layer Chromatography.- Use of a Real-Time, Coupled Assay to Measure the ATPase Activity of DNA Topoisomerase II.- Analysis of Topoisomerase-DNA Interactions by Electrophoretic Mobility Shift Assay.- Filter Binding Assays for Topoisomerase—DNA Complexes.- DNATopoisomerase I-Mediated Nicking of Circular Duplex DNA.- Analysis of Cleavage Complexes Using Reactive Inhibitor Derivatives.- Uncoupling of Topoisomerase-Mediated DNA Cleavage and Religation.- Synthesis and Use of DNA Containing a 5?-Bridging Phosphorothioate as a Suicide Substrate for Type I DNA Topoisomerases.- Isolation of Covalent Enzyme-DNA Complexes.- ICE Bioassay.- DNA-Unwinding Test Using Eukaryotic DNA Topoisomerase I.- Drug—DNA Interactions.- Quinolone Interactions with DNA and DNA Gyrase.- Bactericidal Assays for Fluoroquinolones.- Drug Toxicity in E. coli Cells Expressing Human Topoisomerase I.- Drug-Induced Cytotoxicity in Tissue Culture.- Analysis of Drug Uptake-Flow Cytometry.- Cell-Cycle Analysis of Drug-Treated Cells.- Assaying Drug-Induced Apoptosis.- Single-Strand Conformational Polymorphism Analysis of DNA Topoisomerases I and II.- PCR-Based Cloning of DNA Topoisomerase Genes.- Topoisomerase II-Catalyzed Relaxation and Catenation of Plasmid DNA.- Topoisomerase II-Mediated Cleavage of Plasmid DNA.- Drug-induced Stabilization of Covalent DNA Topoisomerase I-DNA Intermediates.- Studying DNATopoisomerase I-Targeted Drugs In the Yeast.- Yeast Systems for Demonstrating the Targets of Anti-Topoisomerase II Agents.ReviewsThis book completes a comprehensive collection of assays, including the weird and wonderful, that can be used to investigate the enzymology and drug inhibition of topoisomerases...I recommend any students about to undertake a PhD degree in this area to buy a copy. Even those who are well versed in the biology of topoisomerases should consider buying both volumes of this book, they will surely find some things they didn't know - I did! - MicroBiology Today Described by expert experimentalists who have perfected the techniques, these reproducible methods include assays for enzyme-catalyzed DNA relaxation/supercoiling, DNA cleavage, DNA nicking, DNA decatentaion, and ATP hydrolysis. - Anticancer Research An unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs. -Tumori This book completes a comprehensive collection of assays, including the weird and wonderful, that can be used to investigate the enzymology and drug inhibition of topoisomerases...I recommend any students about to undertake a PhD degree in this area to buy a copy. Even those who are well versed in the biology of topoisomerases should consider buying both volumes of this book, they will surely find some things they didn't know - I did! - MicroBiology Today Described by expert experimentalists who have perfected the techniques, these reproducible methods include assays for enzyme-catalyzed DNA relaxation/supercoiling, DNA cleavage, DNA nicking, DNA decatentaion, and ATP hydrolysis. - Anticancer Research An unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs. -Tumori Author InformationTab Content 6Author Website:Countries AvailableAll regions |
||||
