Overview

This book provides methods and clear protocols for the various technologies available to detect, characterize, and study Citrus tristeza virus (CTV), a member of the genus Closterovirus, family Closteroviridae. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this volume reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Citrus Tristeza Virus: Methods and Protocols serves as an ideal guide for plant pathologists, plant virologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease.

Full Product Details

Author:   Antonino F. Catara ,  Moshe Bar-Joseph ,  Grazia Licciardello
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   2015
Weight:   0.500kg
ISBN:  

9781493995608

ISBN 10:   149399560
Pages:   247
Publication Date:   14 August 2020
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. A Short Note on Reflections and Publications on Citrus Tristeza Virus (CTV) Methodologies             Moshe Bar-Joseph   2. A Brief Historical Account of the Family Closteroviridae             Giovanni P. Martelli   3. Phenotyping Biological Properties of CTV Isolates             Marcella Russo and Antonino F. Catara   4. CTV Vectors and Interactions with the Virus and Host Plants             Raymond Yokomi   5. Tissue Print and Squash Captures Real-Time RT-PCR Method for Direct Detection of Citrus Tristeza Virus (CTV) in Plant or Vector Tissues             Mariano Cambra, Eduardo Vidal, Carmen Martínez, and Edson Bertolini   6. Detection of Citrus Tristeza Virus and Co-Infecting Viroids             Maria Saponari, Stefania Zicca, Giuliana Loconsole, Beatriz Navarro, and Francesco Di Serio   7. Assessment of Genetic Variability of Citrus Tristeza Virus by SSCP and CE-SSCP             Elisavet K. Chatzivassiliou and Grazia Licciardello   8. Identification and Characterization of Resistance-Breaking (RB) Isolates of Citrus Tristeza Virus             Maria Saponari, Annalisa Giampetruzzi, Vijayanandraj Selvaraj, Yogita Maheshwari, and Raymond Yokomi   9. Genotyping Citrus Tristeza Virus Isolates by Sequential Multiplex RT-PCR and Microarray Hybridization in a Lab-On-Chip Device             Giuseppe Scuderi, Antonino Catara, and Grazia Licciardello   10. Rapid and Sensitive Detection of Citrus Tristeza Virus Using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) Assay             Dilip Kumar Ghosh, Ashish Warghane, and Kajal Kumar Biswas   11. Amplification and Cloning of Large cDNA Fragments of the Citrus Tristeza Virus Genome             Munir Mawassi, Sabrina Haviv, and Ludmila Meslenin   12. Bioinformatic Tools and Genome Analysis of Citrus Tristeza Virus             Ana Belén Ruiz-García, Rachelle Bester, Antonio Olmos, and Hans Jacob Maree   13. Analysis of Genotype Composition of Citrus Tristeza Virus Populations Using Illumina Miseq Technology             David A. Read and Gerhard Pietersen   14. Citrus Tristeza Virus: Host RNA Silencing and Virus Counteraction             Susana Ruiz-Ruiz, Beatriz Navarro, Leandro Peña, Luis Navarro, Pedro Moreno, Francesco Di Serio, and Ricardo Flores   15. Proteomic Response of Host Plants to Citrus Tristeza Virus             Milena Santos Dória and Carlos Priminho Pirovani   16. Gene Expression in Citrus Plant Cells Using Helios® Gene Gun System for Particle Bombardment             Yosvanis Acanda, Chunxia Wang, and Amit Levy   17. Methods for Producing Transgenic Plants Resistant to CTV             Nuria Soler, Montserrat Plomer, Carmen Fagoaga, Pedro Moreno, Luis Navarro, Ricardo Flores, and Leandro Peña

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