Overview

Cilia are highly conserved organelles that serve motile functions, sensory functions, or both. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport that is essential for the assembly and maintenance of ciliary structures.

Full Product Details

Author:   Stephen M. King (Professor, Department of Molecular Biology and Biophysics Director, Electron Microscopy Facility, University of Connecticut Health Center) ,  Gregory J Pazour
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Edition:   93rd edition
Volume:   93
Dimensions:   Width: 19.10cm , Height: 2.30cm , Length: 23.50cm
Weight:   0.920kg
ISBN:  

9780123813770

ISBN 10:   0123813778
Pages:   416
Publication Date:   05 December 2009
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

1. Manipulating Ciliary Protein-Encoding Genes in Tetrahymena thermophila Drashti Dave, Dorota Wloga, and Jacek Gaertig 2. Approaches for Functional Analysis of Flagellar Proteins in African Trypanosomes Michael Oberholzer, Miguel A. Lopez, Katherine S. Ralston, and Kent L. Hill 3. Tools for Analyzing Intraflagellar Transport in Trypanosomes Daria Julkowska and Philippe Bastin 4. Schmidtea mediterranea: A Model System for Analysis of Motile Cilia Panteleimon Rompolas, Ramila S. Patel-King, and Stephen M. King 5. Targeted Gene Silencing by RNA Interference in Chlamydomonas Eun-Jeong Kim and Heriberto Cerutti 6. Analysis of Cargo Transport by IFT and GFP Imaging of IFT in Chlamydomonas Dennis Diener 7. Genetic and Phenotypic Analysis of Flagellar Assembly Mutants in Chlamydomonas reinhardtii Carlo Iomini, Jacob E. Till, and Susan K. Dutcher 8. Recording and Analyzing IFT in Chlamydomonas Flagella William Dentler, Kristyn VanderWaal, and Mary E Porter 9. Total Internal Reflection Fluorescence (TIRF) Microscopy of Chlamydomonas Flagella Benjamin D. Engel, Karl-Ferdinand Lechtreck, Tsuyoshi Sakai, Mitsuo Ikebe, George B. Witman, and Wallace F. Marshall 10. Purification of IFT Particle Proteins and Preparation of Recombinant Proteins for Structural and Functional Analysis Robert H. Behal, Ewelina Betleja, and Douglas G. Cole 11. Studying Cilia in Zebrafish Iain Drummond 12. Analysis of IFT Kinesins in Developing Zebrafish Cone Photoreceptor Sensory Cilia Christine Insinna, Katherine Luby-Phelps, Brian A. Link, and Joseph C. Besharse 13. Analysis of Intraflagellar Transport in C. elegans Sensory Cilia Limin Hao, Seyda Acar, James Evans, Guangshuo Ou, and Jonathan M. Scholey 14. Functional Genomics of Intraflagellar Transport-Associated Proteins in C. elegans Peter N. Inglis, Oliver E. Blacque, and Michel R. Leroux 15. Generating Conditional Mutants to Analyze Ciliary Functions: The Use of Cre–Lox Technology to Disrupt Cilia in Specific Organs Amber K. O’Connor, Robert A. Kesterson, and Bradley K. Yoder 16. Imaging Intraflagellar Transport in Mammalian Primary Cilia Tatiana Y. Besschetnova, Barnali Roy, and Jagesh V. Shah 17. Analysis of Hedgehog Signaling in Mouse Intraflagellar Transport Mutants Hyuk W. Ko, Aimin Liu, and Jonathan T. Eggenschwiler

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Author Information

Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements.

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