Overview

Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world, present a collection of the state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both the novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays. Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.

Full Product Details

Author:   Michael N. Marsh
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2000
Volume:   41
Dimensions:   Width: 15.20cm , Height: 1.70cm , Length: 22.90cm
Weight:   0.450kg
ISBN:  

9781617371158

ISBN 10:   1617371157
Pages:   288
Publication Date:   09 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Celiac Disease.- Genotyping Methodologies.- From Linkage to Genes.- Linkage and the Transmission Disequilibrium Test in Complex Traits.- Extraction, Separation, and Purification of Wheat Gluten Proteins and Related Proteins of Barley, Rye, and Oats.- Structure Elucidation of Gluten-Derived Peptides by Tandem Mass Spectrometry.- Synthetic Peptide Libraries for T-Cell Epitope Identification.- Characterization of HLA-DQ-Specific Peptide-Binding Motifs.- Studies of Gliadin-Specific T-Cells in Celiac Disease.- Morphometric Analysis of Intestinal Mucosa.- Morphometry of Rectal Mucosa.- Organ Culture of Rectal Mucosa.- In Situ Hybridization.- Measurements of Cytokine mRNA Expression by Quantitative Polymerase Chain Reaction in Studies of Celiac Disease.- Immunohistochemistry in Research and Diagnosis of Celiac Disease.- Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease.- Antiendomysial and Antigliadin Antibody Tests and Diagnosis of Celiac Disease.- Whole Gut Lavage Fluid Analysis.

Reviews

Of interest to gastroenterologists, pathologists and those working in research on celiac disease, a disease linked by a so far unknown mechanism to type 1 diabetes. -Journal of Pediatric Endocrinology & Metabolism

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