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OverviewThis volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Carbohydrate-Based Vaccines: Methods and Protocols aims to be a valuable manual that will facilitate research in the field of carbohydrate-based vaccines. Full Product DetailsAuthor: Bernd LepeniesPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: 1st ed. 2015 Volume: 1331 Dimensions: Width: 17.80cm , Height: 2.00cm , Length: 25.40cm Weight: 7.117kg ISBN: 9781493928736ISBN 10: 1493928732 Pages: 255 Publication Date: 14 July 2015 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Manufactured on demand We will order this item for you from a manufactured on demand supplier. Table of ContentsCarbohydrate-based Vaccines: An Overview.- Glycans as Vaccine Antigens and Adjuvants: Immunological Considerations.- The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens.- Antibody-carbohydrate Recognition from Docked Ensembles using the AutoMap Procedure.- Generation of Monoclonal Antibodies against Defined Oligosaccharide Antigens.- Murine Whole-Blood Opsonophagocytosis Assay to Evaluate Protection by Antibodies Raised Against Encapsulated Extracellular Bacteria.- Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility.- N-Glycosylation Fingerprinting of Viral Glycoproteins by xCGE-LIF.- Temporary Conversion of Protein Amino Groups To Azides: A Synthetic Strategy For Glycoconjugate Vaccines.- Gold Nanoparticles as Carriers For Synthetic Glycoconjugate Vaccines.- Identification and Characterization of Carbohydrate-Based Adjuvants.- Characterization of Carbohydrate Vaccines by NMR Spectroscopy Francesco Berti and Neil Ravenscroft.- Characterisation of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods.- Glycoconjugate Vaccines: The Regulatory Framework.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |