Candida Species: Methods and Protocols

Author:   Richard Calderone
Publisher:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2022
Volume:   2542
ISBN:  

9781071625484


Pages:   390
Publication Date:   26 August 2022
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Candida Species: Methods and Protocols


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Author:   Richard Calderone
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2022
Volume:   2542
Weight:   0.954kg
ISBN:  

9781071625484


ISBN 10:   1071625489
Pages:   390
Publication Date:   26 August 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: The Candida Genome   1. CRISPR-Mediated Genome Editing in the Human Fungal Pathogen C. albicans             Ben A. Evans and Douglas A. Bernstein   2. Plasmid-Based CRISPR–Cas9 Editing in Multiple Candida Species             Lisa Lombardi and Geraldine Butler   3. A Proteomic Approach for the Quantification of Post-Translational Protein Lysine Acetylation in Candida albicans             Raju Shivarathri, Manju Chauhan, Rounik Mazumdar, Phan Canh Trinh, Wolfgang Reiter, Markus Hartl, Karl Kuchler, and Neeraj Chauhan   4. How to Use the Candida Genome Database             Marek S. Skrzypek, Jonathan Binkley, and Gavin Sherlock   5. Sub-Telomeric Chromatin Structure by Chromosome Conformation Capture (3C)-qPCR Methodology in Candida glabrata             Eunice López-Fuentes, Grecia Hernández-Hernández, Alejandro De Las Peñas, and Irene Castaño   6. Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic Analysis in Candida albicans             Virginia Glazer and Damian Krysan   Part II: Antifungal Discovery and Resistance   7. Using Synthetic Genetic Interactions in Candida glabrata as a Novel Method to Detect Genes with Roles in Antifungal Drug Resistance             Jane Usher   8. Identifying Specific Small Molecule-Protein Interactions Using Target Abundance-Based Fitness Screening (TAFiS)             Jessica Regan and Glen E. Palmer   9. High Throughput Phospholipidomics of Candida Cells: From Sample Preparation to Data Analysis             Mohit Kumar, Rajendra Prasad, and Ashutosh Singh   10. Genetic Screens of an Anti-Candida Natural Product Using the Heterozygous     Saccharomyces cerevisiae Mutant Library             Lulu Zhang, Dongmei Li, Richard Calderone, and Xiaodong She   11. Assessing Complex I (CI) Mitochondrial Subunit Protein Functions in Candida albicans             Dongmei Li, Xiaodong She, and Richard Calderone   Part III: Pathogenesis and Virulence   12. In Vitro Biophysical Characterization of Candidalysin: A Fungal Peptide Toxin             Sejeong Lee, Nessim Kichik, Olivia W. Hepworth, Jonathan P. Richardson, and Julian R. Naglik   13. Assessment of cAMP-PKA Signaling in Candida glabrata by FRET-Based Biosensors             Liesbeth Demuyser, Wouter Van Genechten, and Patrick Van Dijck   14. Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anergy             Junko Yano and Paul L. Fidel, Jr.   15. A Simple Method for Growth of Candida albicans Biofilms under Continuous Media Flow and for Recovery of Biofilm Dispersed Cells             Priya Uppuluri   16. A Simple 96 Well Plate-Based Method for Development of Candida Biofilms under Static Conditions             Priya Uppuluri   17. Comparative Analysis of the Fitness of Candida albicans Strains during Colonization of the Mice Gastrointestinal Tract             Daniel Prieto and Jesús Pla   18. Detection and Identification of Candida auris from Clinical Skin Swabs             Milena Kordalewska and David S. Perlin   19. Production and Isolation of the Candida Species Biofilm Extracellular Matrix             Marienella Hereidia and David Andes   Part IV: Communities   20. Characterization of the Effects of Candida Gastrointestinal Colonization on Clostridioides difficile Infection in a Murine Model             Jesús A. Romo and Carol A. Kumamoto   21. Analysis of Gut Microbiome Using Fecal Samples             Ahmed Gamal, Mayyadah Alabdely, Kelly Steller, Hilmi Al-Shakhir, Mohammed Elshaer, and Mahmoud Ghannoum   22. Measurements of Treg Cell Induction by Candida albicans DNA Using Flow Cytometry             Dongmei Li, Jie Cheng, Richard Calderone, and Joseph A. Bellanti   Part V: Immunity   23. Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure             Ebrima Bojang, Rebecca Drummond, and Rebecca A. Hall   24. Isolation, Physicochemical Characterization, Labeling, and Biological Evaluation of Mannans and Glucans             Michael D. Kruppa, Douglas W. Lowman, Harry E. Ensley, Zuchao Ma, Bridget Graves, Jennifer Kintner, Jennifer V. Hall, Tammy R. Ozment, and David L. Williams   25. Understanding the Protective Role of IL-17 during Oropharyngeal Candidiasis             Jessica Saul-McBeth and Heather R. Conti

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