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OverviewEarly approaches towards an understanding of transport at the tonoplast of higher plant cells were based on the kinetics of radio-isotope uptake (Torii and Laties. 1966) and exchange (Pitman, 1963). A variety of other methods became available in due course (reviewed by Clarkson and Luttge, 1984). But even with the most modern developments, the function of. the tonoplast remained in the dark. On the basis of Hitchell's chemiosmotic $heory (Hitchell, 1967), mechanisms of membrane-bound H -translocating ATPases and H -solute co-transport were conceived for the plasmalemma of fungi and plants, based on experiments with intact tissues, cells or protoplasts . With the onset of the era of membrane fractionation it seemed obvious that plasmalemma and tonoplast vlould cause the greatest problems due to the apparent lack of intrinsic markers. Again the situation for the plasmalemma appeared to be favorable; the periodic-phosphotungstic-acid reagent was considered as a specific stain. There was also the opportunity to bind externally applied markers to the plasmalemma prior to cell homogenization. Vacuoles were initially considered as compartments for intracellular excretion or at best as storage compart- ments, but were later understood to have important dynamic functions in cell physiology as a lytic compart- ment comparable to animal lysosomes (Hatile, 1966). The dynamics of molecular functions of the tonoplast itself remained obscure, however, with one exception. Hotivated by the search for a better understanding of latex production by the rubber tree Hevea brasiliensis Hull. -Arg. Full Product DetailsAuthor: B.P. MarinPublisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Imprint: Springer-Verlag Berlin and Heidelberg GmbH & Co. K Edition: Softcover reprint of the original 1st ed. 1985 Dimensions: Width: 17.00cm , Height: 1.50cm , Length: 24.40cm Weight: 0.502kg ISBN: 9783642703225ISBN 10: 3642703224 Pages: 259 Publication Date: 19 November 2011 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Manufactured on demand  We will order this item for you from a manufactured on demand supplier. Table of ContentsCharacteristics of Tonoplast Membranes.- Plant Vacuole Membrane: Structure and Properties (With 9 Figures).- Analytical Characterization of Vacuolar Membranes from Higher Plants (With 11 Figures).- Tonoplast Adenosine-Triphosphatases.- Comparative Analysis of the Properties of Tonoplast-Bound Adenosine-Triphosphatase from Fungi and Higher Plants: Pitfalls and Artifacts in the Search for Vacuolar ATPase and Proton Pumps.- Salt-Stimulated ATPase and PPase Activities Associated With Vacuoles from Higher Plants (with 2 Figures).- Ca2+-ATPases and Their Occurrence in Vacuoles of Higher Plants (With 2 Figures).- Isolation, Properties, and Functions of Tonoplast ATPase from Higher Plants.- Measurement of Protonmotive Potential Difference Across the Tonoplast Membrane.- Membrane Potential Changes in Vacuoles from Storage Roots of Red Beet (With 2 Figures).- Transtonoplast Potential Difference and Surface Potential of Isolated Vacuoles (with 7 Figures).- Critical Evaluation of Methods for Estimating the Vacuolar pH of Plant Cells (With 5 Figures).- A Study of Transport in Tonoplast Vesicles Using Spin-Labelled Probes (With 3 Figures).- The Use of Optical Probes to Monitor the Formation of pH Gradients and Membrane Potential in Tonoplast Membrane Vesicles (With 4 Figures).- Proton Pump Activity and Tonoplast Energization.- The H+-Translocating ATPase in Vacuolar Membranes of Neurospora Crassa (With 2 Figures).- The H+-Translocating ATPase in Vacuolar Membranes of Saceharomyoes Cerevisiae (With 2 Figures)..- The Functioning of Tonoplast H+-Translocating ATPase from Hevea Latex in Physiological Conditions (With 4 Figures).- Proton Pump and ATPase Activities in Tonoplast Vesicles from Storage Tissue of Red Beet (With 4 Figures).- Anion-Sensitive H+-ATPases from Higher Plant Cells: The Role of Chloride in Stimulating Proton Transport (With 3 Figures).- An Electrogenic Proton Pump on the Tonoplast of Acer Pseudoplatanus L. Free Cells and Isolated Vacuoles (With 7 Figures).- Role of Tonoplast Adenosine-Triphosphatase in the Compartmentation of Solutes in Plant Cells.- H+-ATPase and H+/Ion Antiporters of the Vacuolar Membrane of Sacchavomyces Carlsbergensis Yeast (With 7 Figures).- Compartmentation of Solutes and the Role of Tonoplast ATPase in Hevea Latex (With 2 Figures).- Transport of Malic Acid in Cells of CAM Plants.- Transport and Energization in Vacuoles of Sugarcane (With 3 Figures).- Cooperation of a “Davies Type” Biochemical pH-Stat and the Tonoplastic Bioosmotic pH-Stat in the Regulation of the Cytosolic pH of Hevea Latex (With 7 Figures).ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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