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OverviewBenson's Microbiological Applications has been the gold standard of microbiology laboratory manuals for over 30 years. The 59 self-contained, clearly-illustrated exercises, and four-color format makes Microbiological Applications: Laboratory Manual in General Microbiology, the ideal lab manual. Appropriate for either a majors or non-majors lab course, this lab manual assumes no prior organic chemistry course has been taken. Full Product DetailsAuthor: Alfred BrownPublisher: McGraw-Hill Education - Europe Imprint: McGraw-Hill Professional Edition: 12th edition Dimensions: Width: 22.40cm , Height: 2.30cm , Length: 27.20cm Weight: 0.903kg ISBN: 9780073375274ISBN 10: 0073375276 Pages: 480 Publication Date: 16 February 2011 Audience: General/trade , General Replaced By: 0073402419 Format: Spiral bound Publisher's Status: Out of Print Availability: In Print Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock. Table of ContentsPart 1 Microscopy1 Brightfield Microscopy2 Darkfield Microscopy3 Phase-Contrast Microscopy4 Microscopic MeasurementsPart 2 Survey of Microorganisms5 Protozoa, Algae and Cyanobacteria6 Ubiquity of Bacteria7 The Fungi: Molds and YeastsPart 3 Manipulation of Microorganisms8 Aseptic Technique9 Pure Culture TechniquesPart 4 Staining and Observations of Microorganisms10 Smear Preparation11 Simple Staining12 Negative Staining13 Capsular Staining14 Gram Staining15 Spore Staining: Two Methods16 Acid-Fast Staining: Kinyoun Method17 Motility DeterminationPart 5 Culture Methods18 Culture Media Preparation19 Enumeration of Bacteria: The Standard Plate Count20 Slide Culture: FungiPart 6 Bacterial Viruses21 Determination of a Bacteriophage Titer22 Isolation of Phage from Flies23 Phage TypingPart 7 Environmental Influences and Control of Microbial Growth24 Effects of Oxygen on Growth25 Temperature: Effects on Growth26 pH and Microbial Growth27 Water Activity and Osmotic Pressure28 Ultraviolet Light: Lethal Effects29 The Effects of Lysozyme on Bacterial Cells30 Evaluation of Alcohol: Its Effectiveness as an Antiseptic31 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method32 Evaluation of Antiseptics: The Filter Paper Disk Method33 Effectiveness of Hand ScrubbingPart 8 Identification of Unknown Bacteria34 Morphological Study of An Unknown Bacterium35 Cultural Characteristics36 Physiological Characteristics: Oxidation and Fermentation Stet Reactions37 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions38 Physiological Characteristics: Multiple Test Media39 Use of Bergey’s ManualPart 9 Miniaturized Multitest Systems40 Enterobacteriaceae Identification: The API 20E System41 Enterobacteriaceae Identification: The Enterotube II System42 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System43 Staphylococcus Identification: The API Staph SystemPart 10 Applied Microbiology44 Bacterial Food Counts45 Bacteriological Examination of Water: Most Probable Number Determination46 Bacteriological Examination of Water: The Membrane Filter Method47 Reductase Test48 Temperature: Lethal Effects49 Microbial Spoilage of Canned Food50 Microbiology of Alcohol FermentationPart 11 Medical Microbiology51 The Staphylococci: Isolation and Identification52 The Streptococci and Enterococci: Isolation and Identification53 Gram-Negative Intestinal Pathogens54 A Synthetic EpidemicPart 12 Immunology and Serology55 Slide Agglutination Test: Serological Typing56 Slide Agglutination Test for S. aureus57 Slide Agglutination Test for Streptococcus58 Tube Agglutination Test: The Heterophile Antibody Test59 Blood GroupingAppendicesReviewsAuthor InformationProfessor and Chair, Consultant Environmental Microbiology Tab Content 6Author Website:Countries AvailableAll regions |