Overview

Benson's Microbiological Applications has been the gold standard of microbiology laboratory manuals for over 30 years. The 77 self-contained, clearly-illustrated exercises, and four-color format makes Microbiological Applications: Laboratory Manual in General Microbiology, the ideal lab manual. Appropriate for either a majors or non-majors lab course, this lab manual assumes no prior organic chemistry course has been taken.

Full Product Details

Author:   Alfred Brown
Publisher:   McGraw-Hill Education - Europe
Imprint:   McGraw-Hill Professional
Edition:   12th edition
Dimensions:   Width: 22.90cm , Height: 2.50cm , Length: 26.90cm
Weight:   1.082kg
ISBN:  

9780077302139

ISBN 10:   0077302133
Pages:   576
Publication Date:   16 February 2011
Audience:   General/trade ,  General
Replaced By:   0077668022
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Part 1 Microscopy1 Brightfield Microscopy2 Darkfield Microscopy3 Phase-Contrast Microscopy4 Fluorescence Microscopy5 Microscopic MeasurementsPart 2 Survey of Microorganisms6 Protozoa, Algae and Cyanobacteria7 Ubiquity of Bacteria8 The Fungi: Molds and YeastsPart 3 Manipulation of Microorganisms9 Aseptic Technique10 Pure Culture TechniquesPart 4 Staining and Observations of Microorganisms11 Smear Preparation12 Simple Staining13 Negative Staining14 Capsular Staining15 Gram Staining16 Spore Staining: Two Methods17 Acid-Fast Staining: Kinyoun Method18 Motility DeterminationPart 5 Culture Methods19 Culture Media Preparation20 Preparation of Stock Cultures21 Enumeration of Bacteria: The Standard Plate Count22 Slide Culture: FungiPart 6 Bacterial Viruses23 Determination of a Bacteriophage Titer24 Burst Size Determination: A One-Step Growth Curve25 Isolation of Phage from Flies26 Phage Typing Part 7 Environmental Influences and Control of Microbial Growth27 Effects of Oxygen on Growth28 Temperature: Effects on Growth29 pH and Microbial Growth30 Water Activity and Osmotic Pressure31 Ultraviolet Light: Lethal Effects32 The Effects of Lysozyme on Bacterial Cells33 Evaluation of Alcohol: Its Effectiveness as an Antiseptic34 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method35 Evaluation of Antiseptics: The Filter Paper Disk Method36 Effectiveness of Hand ScrubbingPart 8 Identification of Unknown Bacteria37 Morphological Study of An Unknown Bacterium38 Cultural Characteristics39 Physiological Characteristics: Oxidation and Fermentation Stet Reactions40 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions41 Physiological Characteristics: Multiple Test Media42 Use of Bergey’s ManualPart 9 Miniaturized Multitest Systems43 Enterobacteriaceae Identification: The API 20E System44 Enterobacteriaceae Identification: The Enterotube II System45 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System46 Staphylococcus Identification: The API Staph SystemPart 10 Diversity and Environmental Microbiology47 Isolation of an Antibiotic Producer: The Actinomyces48 Nitrogen Cycle: Ammonification49 Symbiotic Nitrogen Fixation: Rhizobium50 Free-Living Nitrogen Fixation: Azotobacter51 Denitrification: Paracoccus denitrificans52 The Winogradsky Column53 Purple Nonsulfur Photosynthetic Bacteria54 Sulfate Reducing Bacteria: Desulfovibrio55 Bacterial Commensalism56 Bacterial Synergism57 Microbial Antagonism Part 11 Applied Microbiology58 Bacterial Food Counts59 Bacteriological Examination of Water: Most Probable Number Determination60 Bacteriological Examination of Water: The Membrane Filter Method61 Reductase Test62 Temperature: Lethal Effects63 Microbial Spoilage of Canned Food64 Microbiology of Alcohol FermentationPart 12 Bacterial Genetics and Biotechnology65 Mutant Isolation by Replica Plating66 Bacterial Transformation67 Polymerase Chain Reaction for Amplifying DNA68 Plasmid IsolationPart 13 Medical Microbiology69 The Staphylococci: Isolation and Identification70 The Streptococci and Enterococci: Isolation and Identification71 Gram-Negative Intestinal Pathogens72 A Synthetic EpidemicPart 14 Immunology and Serology73 Slide Agglutination Test: Serological Typing74 Slide Agglutination Test for S. aureus75 Slide Agglutination Test for Streptococcus76 Tube Agglutination Test: The Heterophile Antibody Test77 Blood Grouping Appendices

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Professor and Chair, Consultant Environmental Microbiology

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