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Author:   Martha R. J. Clokie ,  Andrew Kropinski ,  Rob Lavigne
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1898
Weight:   0.666kg
ISBN:  

9781493989393

ISBN 10:   1493989391
Pages:   216
Publication Date:   20 December 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

PhageFISH for Monitoring Phage Infections at Single Cell Level.- Fluoromycobacteriophages for Drug Susceptibility Testing (DST) of Mycobacteria.- Engineering Bacteriophage-Based Biosensors.- Introduction of Phage Genome into Escherichia coli by Electroporation.- Site-Specific Mutagenesis of Bacillus subtilis Phage SPO1.- Genetic Manipulation of Lytic Bacteriophages with BRED: Bacteriophage Recombineering of Electroporated DNA.- Isolation of Competitive Phage Display-Modified Bacteriophage T4 with Affinity Chromatography.- Immobilization of Intact Phage and Phage-Derived Proteins for Detection and Biocontrol Purposes.- Peptidoglycan Hydrolytic Activity of Bacteriophage Lytic Proteins in Zymogram Analysis.- Analyzing Phage-Host Protein-Protein Interactions using Strep-tag® II Purifications.- Techniques to Assess Phage/Biofilm Interaction.- Screening for Growth-Inhibitory ORFans in Pseudomonas aeruginosa-Infecting Bacteriophages.- The Use of the greater Wax Moth Larvae (Galleria mellonella) as an Alternative Animal Infection Model for the Analysis of Bacterial Pathogenesis.- Use of a Silkworm Larva Model in Phage Therapy Experiments.- Exploitation of Drosophila Infection models to Evaluate Antibacterial Efficacy of Phages.- Duckweed (Lemna minor) and Alfalfa (Medicago sativa) as Bacterial Infection Model Systems.- Quantitating Phage Efficacy in ready-To-Eat Meats.- Use of a Chicken Embryo Lethality Assay to Assess the Efficacy of Phage Therapy.

Reviews

The book describes the functional elucidation of bacteriophages in the current era, as well as the drive towards application-driven research. ... this is a very interesting resource for protocols regarding phages and their use for therapy. I am going to include it in my reference material for my own students working on phages in relation to the gut microbiota. (Koen Venema, Beneficial Microbes, Vol. 10 (3), 2019)

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