Overview

This detailed volume explores the field of bacterial virulence and the effort to understand how microbial interaction with a host results in the pathology of a specific disease. This collection of selected protocols includes advanced molecular biology and bioinformatics methods, cell culture and organoid models of infection, as well as in vivo infection models that are useful to study the interaction of pathogens with plants, insects, avian, and mammalian hosts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and beneficial, Bacterial Virulence: Methods and Protocols serves as an ideal guide for researchers seeking to promote and further develop the exciting and continuously evolving field of bacterial virulence. Chapter 19 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Full Product Details

Author:   Ohad Gal-Mor
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2427
Weight:   0.729kg
ISBN:  

9781071619704

ISBN 10:   1071619705
Pages:   267
Publication Date:   27 May 2022
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Molecular Biology and Bioinformatics Methods   1. Generation of Markerless Gene Deletion Mutants in Listeria monocytogenes Using a Mutated PheS for Counterselection             Shai Ran Sapir, Etai Boichis, and Anat A. Herskovits   2. A Rapid Fluorescence-Based Screen to Identify Regulators and Components of Interbacterial Competition Mechanisms in Bacteria             Daniel Tchelet and Dor Salomon   3. Predicting Type III Effector Proteins Using the Effectidor Web-Server             Naama Wagner, Doron Teper, and Tal Pupko   4. Assay for Type III Secretion in Escherichia coli             Bosko Mitrovic and Neta Sal-Man   5. Profiling of Secreted Type 3 Secretion System Substrates by Salmonella enterica             Rivka Shem-Tov and Ohad Gal-Mor   Part II: Cell Culture and Organoid Models of Infection   6. Analysis of SPI-1 Dependent Type III Secretion and Injection Using a NanoLuc Luciferase-Based Assay             Sara Vilela Pais, Sibel Westerhausen, Erwin Bohn, and Samuel Wagner   7. Mycobacterium tuberculosis Infection of THP-1 Cells: A Model for High Content Analysis of Intracellular Growth and Drug Susceptibility             Leah Rankine-Wilson, Céline Rens, Henok Asfaw Sahile, and Yossef Av-Gay   8. Bone Marrow-Derived Macrophage (BMDM) Infection by Listeria monocytogenes             Etai Boichis, Shai Ran Sapir, and Anat A. Herskovits   9. Preparation and Inflammasome Activation of Murine Bone Marrow-Derived and Resident Peritoneal Macrophages             Izumi Sasaki and Tsuneyasu Kaisho   10. Flow Cytometry-Based Single Cell Analyses of Bacterial Adaption to Intracellular Environments             Marc Schulte and Michael Hensel   11. Quantification of Microbial Fluorescent Sensors during Live Intracellular Infections             Erez Mills and Erik Petersen   12. Dissecting Human Blood Immune Cells Response to Intracellular Infection Using Single Cell RNA-Sequencing             Shelly Hen-Avivi and Roi Avraham   13. Salmonella enterica Infection of Human and Mouse Colon Organoid-Derived Monolayers             Erin C. Boyle, Eva J. Wunschel, and Guntram A. Grassl   Part III: In Vivo Models of Infection   14. Analysis of Salmonella enterica Adhesion to Leaves of Corn Salad or Lettuce             Laura Elpers and Michael Hensel   15. Methods for Using the Galleria mellonella Invertebrate Model to Probe Enterococcus faecalis Pathogenicity             Ling Ning Lam, Debra N. Brunson, Jessica K. Kajfasz, and José A. Lemos   16. Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis             Miriam Ravins, Poornima Ambalavanan, Debabrata Biswas, Rachel Tan Ying Min, Kimberly Lim Xuan Zhen, Yael Kaufman, Aparna Anand, Abhinay Sharma, and Emanuel Hanski   17. Mouse Model to Study Salmonella-Induced Colitis             Katrin Ehrhardt and Guntram A. Grassl   18. Analysis of Salmonella Typhi Pathogenesis in a Humanized Mouse Model             Taylor A. Stepien, Stephen J. Libby, Joyce E. Karlinsey, Michael A. Brehm, Dale L. Greiner, Leonard D. Shultz, Thea Brabb, and Ferric C. Fang   19. In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella             Michaël Koczerka, Isabelle Lantier, Anne Pinard, Marie Morillon, Justine Deperne, Ohad Gal-Mor, Olivier Grépinet, and Isabelle Virlogeux-Payant   20. Two In Vivo Models to Study Salmonella Asymptomatic Carrier State in Chicks             Philippe Velge, Pierrette Menanteau, Thierry Chaumeil, Emilie Barilleau, Jérôme Trotereau, and Isabelle Virlogeux-Payant

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