Overview

This volume discusses various methods and protocols used for the experimentation of a wide range of bacterial species, such as Streptococcus pyogenes, Staphylococcus aureus, Streptococcus pneumonia, Listeria monocytogenes, and Mycobacterium marinum. Bacterial Pathogens: Methods and Protocols is divided into 6 parts: Part 1 describes different approaches to identifying and characterizing bacterial effector molecules; Part 2 deals with structural biology of bacterial pathogenesis and how to overcome folding and stability problems with recombinantly expressed proteins; Part 3 details methodology that identifies bacteria in complex communities and how genomes of bacterial pathogens have evolved; Part 4 reflects on the rapid development of advanced imaging techniques that address questions about molecular properties of individual live bacteria, ultrastructure of surfaces, and subcellular localization of bacterial proteins; Part 5 describes methods from in vitro and in vivo modeling of bacterial infections; and Part 6 explores how bacterial pathogens are the true experts of the immune system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Bacterial Pathogens: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.

Full Product Details

Author:   Pontus Nordenfelt ,  Mattias Collin
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2017
Volume:   1535
Dimensions:   Width: 17.80cm , Height: 2.00cm , Length: 25.40cm
Weight:   0.706kg
ISBN:  

9781493982684

ISBN 10:   1493982680
Pages:   357
Publication Date:   07 July 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Protein-Based Strategies to Identify and Isolate Bacterial Virulence Factors.- Analysis of Bacterial Surface Interactions with Mass Spectrometry-Based Proteomics.- Differential Radial Capillary Action of Ligand Assay (DRaCALA) for High-Throughput Detection of Protein-Metabolite Interactions in Bacteria.- Identifying Bacterial Immune Evasion Proteins using Phage Display.- Competition for Iron between Host and Pathogen: A Structural Case Study on Helicobacter pylori.- Common Challenges in Studying the Structure and Function of Bacterial Proteins: Case Studies from Helicobacter pylori.- Development of a Single Locus Sequence Typing (SLST) Scheme for Typing Bacterial Species Directly from Complex Communities.- Reconstructing the Ancestral Relationships between Bacterial Pathogen Genomes.- Making Fluorescent Streptococci and Enterococci for Live Imaging.- Computer Vision-Based Image Analysis of Bacteria.- Assessing Vacuolar Escape of Listeria Monocytogenes.- Immobilization Techniques of Bacteria for Live Super-Resolution Imaging using Structured Illumination Microscopy.- Negative Staining and Transmission Electron Microscopy of Bacterial Surface Structures.- Detection of Intracellular Proteins by High-Resolution Immunofluorescence Microscopy in Streptococcus pyogenes.- Antibody Guided Molecular Imaging of Infective Endocarditis Infection.- The Zebrafish as a Model for Human Bacterial Infections.- Determining Platelet Activation and Aggregation in Response to Bacteria.- Killing Bacteria with Cytotoxic Effector Proteins of Human Killer Immune Cells: Granzymes, Granulysin and Perforin.- In Vitro and In Vivo Biofilm Formation by Pathogenic Streptococci.- Murine Mycobacterium marinum Infection as a Model for Tuberculosis.- Generating and Purifying Fab Fragments from Human and Mouse IgG using the Bacterial Enzymes IdeS, SpeB and Kgp.- Measuring Antibody Orientation at the Bacterial Surface.- Towards Clinical use of the IgG Specific Enzymes IdeS and EndoS against Antibody-Mediated Diseases.

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