Overview

For both volumes: Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.

Full Product Details

Author:   Shaying Zhao ,  Marvin Stodolsky
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2004
Volume:   256
Dimensions:   Width: 15.20cm , Height: 1.90cm , Length: 22.90cm
Weight:   0.507kg
ISBN:  

9781617372896

ISBN 10:   1617372897
Pages:   326
Publication Date:   10 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Use of BAC End Sequences for SNP Discovery.- Exon Trapping for Positional Cloning and Fingerprinting.- Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column.- BAC Microarray-Based Comparative Genomic Hybridization.- Large DNA Transformation in Plants.- Retrofitting BACs With a Selectable Marker for Transfection.- BAC Modification Using a RecA Expressing Shuttle Vector System.- Bacterial Artificial Chromosome Engineering.- ET Recombination.- BAC Engineering for the Generation of ES Cell-Targeting Constructs and Mouse Transgenes.- Microinjection of BAC DNA into the Pronuclei of Fertilized Mouse Oocytes.- Generation of BAC Transgenic Mice.- BAC Rescue.- Herpesviruses.- Cloning of ?-Herpesvirus Genomes as Bacterial Artificial Chromosomes.- Construction of HSV-1 BACs and Their Use for Packaging of HSV-1-Based Amplicon Vectors.- Mutagenesis of Viral BACs With Linear PCR Fragments (ET Recombination).- Mutagenesis of Herpesvirus BACs by Allele Replacement.- Herpesvirus Genome Mutagenesis by Transposon-Mediated Strategies.- Protective DNA Vaccination by Particle Bombardment Using BAC DNA Containing a Replication-Competent, Packaging-Defective Genome of Herpes Simplex Virus Type I.

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