Overview

This volume provides basic and advanced laboratory protocols for the non-specialist and the experienced researcher. Chapters are divided into three parts covering synthesis and characterization of AMPs, studying the interaction of AMPs with model systems or bacteria, and assaying selected biological activities of AMPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Antimicrobial Peptides: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Full Product Details

Author:   Paul R. Hansen
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2017
Volume:   1548
Weight:   0.861kg
ISBN:  

9781493982875

ISBN 10:   1493982877
Pages:   439
Publication Date:   12 July 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Properties, Design, Synthesis and Characterization of AMPs 1. Antimicrobial Peptides: An Introduction Evan F. Haney, Sarah C. Mansour, and Robert E.W. Hancock   2. Tools for Designing Amphipathic Helical Antimicrobial Peptides Davor Juretić, Damir Vukičević, and Alessandro Tossi   3. Chemical Synthesis of Antimicrobial Peptides Lena Münzker, Alberto Oddo and Paul R. Hansen   4. Microwave-Assisted Synthesis of Antimicrobial Peptides Suhas Ramesh, Beatriz G. de la Torre, Fernando Albericio, Hendrik G. Kruger, and Thavendran Govender   5. High Performance Liquid Chromatography and Mass Spectrometry-Based Design of Protolytically Stable Antimicrobial Peptides Mojtaba Bagheri and Robert E. W. Hancock   6. Determination of Structure and Micellar Interactions of Small Antimicrobial Peptides by Solution-State NMR Reinhard Wimmer and Lars Erik Uggerhøj   7. Mass Spectrometry Approaches for Determining the Structure of Antimicrobial Peptides Jiongyu Liu and Jianping Jiang   Part II: Studying Interactions of AMPs with Model Membranes and Bacteria 8. Molecular Dynamics Simulation and Analysis of the Antimicrobial Peptides-Lipid Bilayers Interactions Shima Arasteh and Mojtaba Bagheri   9. Calorimetry Methods to Study Membrane Interactions and Perturbations Induced by Antimicrobial Host Defense Peptides Mauricio Arias, Elmar J. Prenner and Hans J. Vogel   10. Fluorescence and Absorbance Spectroscopy Methods to Study Membrane Perturbations by Antimicrobial Host Defense Peptides Mauricio Arias and Hans J. Vogel   11. Applying Fluorescence Correlation Spectroscopy to Investigate Peptide-Induced Membrane Disruption Kasper Kristensen, Jonas R. Henriksen, and Thomas L. Andresen   12. Macromolecule Biosynthesis Assay and Fluorescence Spectroscopy Methods to Explore Antimicrobial Peptide Mode(s) of Action Bimal Jana, Kristin Renee Baker, and Luca Guardabassi   13. Using Confocal Microscopy and Computational Modeling to Investigate the Cell Penetrating Properties of Antimicrobial Peptides Gabriel Del Rio, Edda Klipp, and Andreas Herrmann 14. Atomic Force Microscopy Study of the Interactions of Indolicidin with Model Membranes and DNA Peter Fojan and Leonid Gurevich   15. Protocols for studying the interaction of MSI-78 with the Membranes of Whole Gram-positive and Gram-negative Bacteria by NMR Nury P. Santisteban , M. R. Morrow, and  V. Booth   16. Preparation of membrane models of Gram-negative bacteria and their interaction with Antimicrobial Peptides studied by CD and NMR Rickey P. Hicks   17. Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells using Circular Dichroism Concetta Avitabile, Luca Domenico D’Andrea and Alessandra Romanelli   18. Studying the Mechanism of Membrane Permeabilization induced by Antimicrobial Peptides using Patch-clamp Techniques Giorgio Rispoli   19. Assays for Identifying Inducers of the Antimicrobial Peptide LL-37 Frank Nylen, Peter Bergman, Gudmundur H. Gudmundsson, and Birgitta Agerberth   20. Methods for Elucidating the Mechanism of Action of Proline-rich and other Non-lytic Antimicrobial Peptides Monica Benincasa, Giulia Runti, Mario Mardirossian, Renato Gennaro,and Marco Scocchi  21. The Interaction of Antimicrobial Peptides with the Membrane and Intracellular Targets of Staphylococcus Aureus Investigated by ATP-leakage, DNA-binding Analysis and the Expression of a LexA Controlled Gene, recA Sanne Gottschalk and Line Elnif Thomsen   Part III: Assaying Selected Biological Activities of AMPs   22. Methods for Investigating Biofilm Inhibition and Degradation by Antimicrobial Peptides Li-av Segev-Zarko and Yechiel Shai   23. Protocols for Studying Inhibition and Eradication of Bacterial Biofilms by Antimicrobial Peptides Vijayalekshmi Sarojini<   24. Protocols for Studying Antimicrobial Peptides (AMPs) as Anti-cancer Agents Laurence Madera and David W. Hoskin   25. Using Oral and Colon Cancer Cells for studying the Anti-cancer properties of Antimicrobial peptides Teerakul Arpornsuwan, Wimolpak Sriwai, Hathaitip Sritanaudomchai, and Sittiruk Roytrakul   26. Using Disease-Associated Enzymes to Activate Antimicrobial Peptide Prodrugs <Éanna B. Forde, Graeme Kelly, Hisham Makki, Zahraa Al-Sharshahi, Deirdre Fitzgerald-Hughes, and Marc Devocelle   27. Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics: LPS and LTA Neutralization Sarah Line Skovbakke and Henrik Franzyk  28. Protocols for Screening Antimicrobial Peptides That Influence Virulence Gene Expression in Staphylococcus aureus Martin Saxtorph Bojer, Mara Baldry, and Hanne Ingmer   29. Methods for in vitro Analysis of Antimicrobial Activity and Toxicity of Anti-keratitis Peptides: Bacterial Viability in tears, MTT and TNF-a release assays Floriana Cappiello, Bruno Casciaro, Satya Sree Kolar, Hasna Baidouri, Alison McDermott, and Maria Luisa Mangoni   30. Methods for In Vivo/ex-vivo Analysis of Antimicrobial Peptides in Bacterial Keratitis: siRNA Knockdown, Colony Counts, Myeloperoxidase, Immunostaining, and RT-PCR Assays Satya Sree Kolar, Hasna Baidouri, Maria Lusia Mangoni, and Alison M. McDermott   31. Haemolytic Activity of Antimicrobial Peptides Alberto Oddo and Paul R. Hansen

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