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OverviewThis volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies. Antibiotics: Methods and Protocols guides readers through chapters on production and design, mode of action, and response and susceptibility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Antibiotics: Methods and Protocols aims to inspire scientific work in the exciting field of antibiotic research. Full Product DetailsAuthor: Peter SassPublisher: Humana Press Inc. Imprint: Humana Press Inc. Edition: 1st ed. 2017 Volume: 1520 Dimensions: Width: 17.80cm , Height: 2.20cm , Length: 25.40cm Weight: 8.489kg ISBN: 9781493966325ISBN 10: 1493966324 Pages: 358 Publication Date: 22 November 2016 Audience: Professional and scholarly , Professional & Vocational Format: Hardback Publisher's Status: Active Availability: Manufactured on demand We will order this item for you from a manufactured on demand supplier. Table of ContentsAntibiotics - Precious Goods in Changing Times.- Mining Bacterial Genomes for Secondary Metabolite Gene Clusters.- Production of Antimicrobial Compounds by Fermentation.- Structure Elucidation of Antibiotics by NMR Spectroscopy.- Computer-Aided Drug Design Methods.- Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents.- Application of a Bacillus subtilis Whole-cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds.- Determination of Bacterial Membrane Impairment by Antimicrobial Agents.- Mass-sensitive Biosensor Systems to Determine the Membrane Interaction of Analytes.- Measurement of Cell Membrane Fluidity by Laurdan GP:Fluorescence Spectroscopy and Microscopy.- In vitro Assays to Identify Antibiotics Targeting DNA Metabolism.- Fluorescence-based real-time Activity Assays to Identify RNase P inhibitors.- Reporter Gene-based Screening for TPP Riboswitch Activators.- Cell-based Fluorescent Screen to Identify Inhibitors of Bacterial Translation Initiation.-Bacterial Histidine Kinases: Overexpression, Purification, and Inhibitor Screen.- Expression Profiling of Antibiotic Resistant Bacteria Obtained by Laboratory Evolution.- Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress Research.- Label-Free Quantitation of Ribosomal Proteins from Bacillus Subtillis for Antibiotic Research.- Functional Metagenomics to Study Antibiotic Resistance.- Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains using High Throughput Sequencing.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |