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OverviewThe present doctoral thesis provides insights into two conventional labeling techniques used in the studies of microbial metabolism - the 13C and fluorescence labeling. Application of both methods on yeast as a model organism is illustrated by two individual research topics, such as metabolic modeling and visualization of stress response by expression of GFP-tagged heat shock protein Hsp12p, respectively. The dissertation begins with a review of the yeast metabolism and metabolic network, discusses the technical details of the respective labeling experiments and concludes with analysis of the experimental results. Special emphasis is given on the set-up of advanced continuous cultivation techniques (D-stat and auxo-accelerostat), used in the respective labeling experiments. The text is for academic researchers and students in microbiology and molecular biotechnology field. Full Product DetailsAuthor: Ildar Nisamedtinov , Nisamedtinov IldarPublisher: LAP Lambert Academic Publishing Imprint: LAP Lambert Academic Publishing Dimensions: Width: 15.20cm , Height: 0.40cm , Length: 22.90cm Weight: 0.104kg ISBN: 9783838302362ISBN 10: 3838302362 Pages: 64 Publication Date: 05 June 2009 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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